| Johns Hopkins University | Education | $517,434,329 |
| National Philanthropic Tr | Public, societal benefit | $249,117,686 |
| Lucas Museum of Narrative Art | Arts, culture & humanities | $165,000,000 |
| The 85 Fund | Human services | $160,000,000 |
| Renaissance Charitable Foundation Inc | Public, societal benefit | $144,682,101 |
| Fidelity Investments Charitable Gift Fund | Public, societal benefit | $127,700,997 |
| The Board of Trustees of the Leland Stanford Junior University | Education | $90,410,606 |
| University of California San Francisco Foundation | Education | $68,713,740 |
| The Church of Jesus Christ of Latter-day Saints | Religion related | $57,173,550 |
| Global Support and Development | Human services | $41,750,000 |
| Vital Strategies Inc | International, foreign affairs | $34,969,000 |
| Broad Institute Inc | Health | $32,285,000 |
| California Institute of Technology | Education | $31,532,760 |
| University of California Berkeley Foundation | Education | $30,879,662 |
| Impactassets Inc | Public, societal benefit | $30,234,867 |
| Medecins Sans Frontieres USA Inc | International, foreign affairs | $28,779,499 |
| Rockefeller Philanthropy Advisors Inc | Public, societal benefit | $25,937,050 |
| Princeton University | Education | $25,464,226 |
| Mayo Clinic | Health | $25,117,951 |
| President and Fellows of Harvard College | Education | $24,339,910 |
| Morgan Stanley Global Impact Funding Trust Inc | Public, societal benefit | $22,012,665 |
| University of Arizona Foundation | Education | $19,815,184 |
| Brown University of Providence | Public, societal benefit | $16,294,945 |
| Trustees of Boston University | Education | $15,352,768 |
| Dechomai Asset Trust | Public, societal benefit | $15,063,017 |
| Trustees of Dartmouth College | Education | $14,680,543 |
| Cornell University | Education | $14,508,780 |
| New Venture Fund | Environmental and animals | $12,836,950 |
| Daniel R Sayre Memorial Foundation Inc | Human services | $12,836,666 |
| Murmuration Research Institute Inc | Public, societal benefit | $12,500,000 |
| Young Life | Human services | $12,402,373 |
| George Lucas Educational Foundation | Education | $12,135,000 |
| Digital Harbor Foundation Inc | Education | $12,091,000 |
| American National Red Cross | Human services | $12,013,035 |
| University of Chicago | Education | $11,894,303 |
| American Endowment Foundation | Public, societal benefit | $11,590,765 |
| Benedictine College | Education | $11,552,700 |
| National Christian Charitable Foundation | Religion related | $11,191,439 |
| St Jude Childrens Research Hospital Inc | Health | $11,067,923 |
| Word of Life Foundation Inc | Religion related | $11,010,000 |
| Medical Debt Resolution Inc | Human services | $10,925,590 |
| World Central Kitchen Incorporated | International, foreign affairs | $10,573,403 |
| Kansas State University Foundation | Education | $10,311,487 |
| Kansas Athletics Incorporated | Education | $10,004,850 |
| Gs Donor Advised Philanthropy Fund For Wealth Management Inc | Public, societal benefit | $9,812,305 |
| Mayo Clinic | Health | $9,733,520 |
| Spirit of America Worldwide | International, foreign affairs | $9,687,918 |
| Regents of the University of Michigan | Education | $9,573,183 |
| Nature Conservancy | Environmental and animals | $8,927,872 |
| United States Fund For Unicef | International, foreign affairs | $8,868,644 |
| Samaritans Purse | Religion related | $8,850,780 |
| Americans For Oxford Inc | Education | $8,747,657 |
| Bank of America Charitable Gift Fund | Public, societal benefit | $8,734,948 |
| Trustees of the University of Pennsylvania | Education | $8,680,471 |
| Yale University | Education | $8,670,723 |
| University of Texas At Austin | Education | $8,636,413 |
| Georgetown University | Education | $8,572,470 |
| Waimea Malama Inc | Environmental and animals | $8,525,000 |
| World Vision | International, foreign affairs | $8,140,687 |
| University of Illinois Foundation | Education | $7,962,005 |
| San Francisco Foundation | Public, societal benefit | $7,947,798 |
| Kyiv School of Economics | Education | $7,816,425 |
| Mission Possible Partnership USA Inc | Environmental and animals | $7,700,000 |
| New Schools For Baton Rouge | Education | $7,590,000 |
| Massachusetts Institute of Technology | Education | $7,581,047 |
| United Jewish Appeal Federation of Jewish Philanthropies of Ny Inc | Public, societal benefit | $7,456,891 |
| The Clear Fund | Public, societal benefit | $7,443,568 |
| Compassion International Incorporated | International, foreign affairs | $7,431,259 |
| Chesapeake Conservancy Inc | Environmental and animals | $7,414,450 |
| Duke University | Education | $7,317,005 |
| University of Washington Foundation | Education | $7,221,436 |
| International Rescue Committee Inc | Human services | $7,207,991 |
| Rest of World Media Inc | Public, societal benefit | $7,093,000 |
| Power To the Patients | Health | $7,000,000 |
| California College of the Arts | Education | $6,994,850 |
| The Community Foundation For Greater Atlanta Inc | Public, societal benefit | $6,937,800 |
| Convergent Research Inc | Public, societal benefit | $6,750,000 |
| Boys & Girls Clubs of America | Human services | $6,681,445 |
| Hawaii Community Foundation | Public, societal benefit | $6,677,158 |
| Campus Crusade For Christ Inc | Religion related | $6,613,205 |
| Science For America Inc | Public, societal benefit | $6,600,000 |
| Christian Community Foundation Inc | Public, societal benefit | $6,560,051 |
| Washington University | Education | $6,495,452 |
| The Nueva School | Education | $6,397,775 |
| Trustees of Columbia University | Education | $6,301,820 |
| The Catholic Association Foundation | Religion related | $6,250,000 |
| Stephen Siller Tunnel To Towers Foundation | Education | $6,216,653 |
| First Nation Group Foundation Inc | Public, societal benefit | $6,196,000 |
| Donors Fund Inc | Public, societal benefit | $6,130,332 |
| Womens Leadership Center At Williams Bay Nfp | Education | $6,100,000 |
| Planned Parenthood Federation of | Health | $6,041,077 |
| First Serve - Nm Inc | Human services | $6,020,000 |
| Santa Fe Institute | Public, societal benefit | $6,016,250 |
| University of Montana Foundation | Education | $5,930,975 |
| P E F Israel Endowment Funds Inc | Public, societal benefit | $5,883,400 |
| Second Harvest of Silicon Valley | Human services | $5,836,492 |
| Friends of the Israel Defense Forces | Human services | $5,832,838 |
| Celebration Church | Religion related | $5,684,200 |
| Burning Man Project | Public, societal benefit | $5,607,800 |
| Pga Tour First Tee Foundation Inc | Human services | $5,540,500 |